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Title
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Purification, crystallisation and preliminary X-ray studies on avian pancreatic polypeptide.
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Authors
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S.P.Wood,
J.E.Pitts,
T.L.Blundell,
I.J.Tickle,
J.A.Jenkins.
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Ref.
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Eur J Biochem, 1977,
78,
119-126.
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PubMed id
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Abstract
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A pancreatic polypeptide with some hormonal properties has been purified from
chicken and turkey pancreas using acid-ethanol extraction, gel filtration and
anion-exchange chromatography. The material has been crystallised. The crystals
are monoclinic with space group C2. Preliminary isomorphous replacement
experiments have so far provided a single-site derivative with Hg(NO3)2. A
low-resolution electron density map phased with this derivative using anomalous
scattering considered together with Patterson function calculations suggest that
the molecules are partly helical and are arranged as a compact dimer about the
crystallographic two-fold axis. The structure and association of these molecules
are compared with those of insulin and glucagon, pancreatic protein and
polypeptide hormones respectively, which have been studied in great detail.
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