 |
|
Title
|
|
Maltodextrin-binding protein hybrids carrying epitopes from the preS2 region of hepatitis B virus: expression, antibody-binding and preliminary crystallographic studies.
|
|
|
Authors
|
|
B.Vulliez-le Normand,
F.A.Saul,
P.Martineau,
F.Lema,
M.Hofnung,
G.A.Bentley.
|
|
|
Ref.
|
|
Protein Eng, 1997,
10,
175-180.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Five hybrid constructions of maltodextrin-binding protein (MBP), each containing
an inserted epitope(s) from the preS2 region of the envelope proteins of
hepatitis B virus (HBV), have been expressed. The anti-preS2 monoclonal antibody
S2.3 was shown to cross-react with the MBP hybrid constructions, demonstrating
that the epitopes presented by these recombinant proteins mimic the antigenic
behaviour of the native viral antigen. In addition, all five hybrid proteins
have been crystallized. Preliminary structural solutions obtained by molecular
replacement indicate that the native conformation of MBP is preserved in the
hybrid constructions despite the significant length of the epitope insertions.
|
|
|
|