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Title
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Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1.
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Authors
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Y.Kurihara,
T.Nagata,
T.Imai,
A.Hiwatashi,
M.Horiuchi,
S.Sakakibara,
M.Katahira,
H.Okano,
S.Uesugi.
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Ref.
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Gene, 1997,
186,
21-27.
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PubMed id
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Abstract
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mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the
developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA
recognition motifs (RRMs). In this study, we found that the N-terminal RRM of
m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar
to that of the full-length m-Msi-1 protein characterized previously. The
C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the
circular dichroism (CD) spectra of the two RRMs showed that the alpha-helical
content of MMA is significantly higher than that of MMB, indicating that some
differences in the secondary structure may be responsible for the distinct RNA
binding properties of MMA and MMB.
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