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The complete nucleotide sequence of the putative fiber protein of a porcine
adenovirus isolate, NADC-1, was determined. The coding sequence for the fiber
protein was found to be 2112 nucleotides, predicting a 703 amino acid protein
with a calculated molecular mass of 76,681 Da. The coding sequence is located
between 86 and 92.5 map units. A polyadenylation signal was found 44 bases
downstream of the stop codon. Northern hybridization analysis identified a band
at 2.4 kb, which likely includes the primary transcript plus a tripartite leader
(typically found with adenovirus transcripts) and a polyA(+) tail. The predicted
NADC-1 fiber protein was found to have a tail domain comparable in size and
sequence to most adenovirus fiber proteins, a comparatively short shaft region,
and a head region that was more than twice as large as that of other adenovirus
fiber proteins. Each of the three structural domains (tail, shaft, and head) of
the predicted NADC-1 fiber protein was found to be most similar to the
corresponding domain of a different mammalian adenovirus. The NADC-1 fiber head
contained an RGD sequence, a motif that is found in the penton protein of other
adenoviruses. Furthermore, the predicted amino acid sequence of the C-terminal
half of the NADC-1 fiber head has significant homology to S-lectin proteins, a
characteristic not shared with other adenovirus fiber proteins. Thus the
predicted amino acid sequence of the NADC-1 fiber head is unique among
adenoviruses for which the sequence of the fiber protein is known.
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