 |
|
Title
|
|
Deduced amino acid sequence of 2S storage protein from Brassica species and their conserved structural features.
|
|
|
Authors
|
|
J.Dasgupta,
S.Dasgupta,
S.Ghosh,
B.Roy,
R.K.Mandal.
|
|
|
Ref.
|
|
Indian J Biochem Biophys, 1995,
32,
378-384.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
2S seed storage albumin coding regions from five Brassica species, namely
Brassica campestris, B. oleracea, B. nigra, B. juncea, and B. carinata have been
cloned by PCR amplification of genomic DNA using oligonucleotide primers and
their nucleotide sequences have been determined. These sequences showed more
than 85% homology amongst themselves and considerable homology with some other
crucifer 2S protein coding sequences. The deduced amino acid sequences showed
more homology due to some inconsequential mutations in codons without changing
the amino acids. Computer analysis of the protein sequences for possible
secondary structure revealed a high degree of conservation of hydrophilic and
hydrophobic domains and the invariant positions of cysteine residues. Unrooted
phylogenic tree based on the coding region of 2S albumin from different Brassica
species cloned by us and published sequences from other Cruciferae indicated
that these genes originated before the evolutionary divergence of different
Brassica species and were conserved due to some stringent structural and
functional features required for seed metabolism.
|
|
|
|