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Title
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Cloning, characterization, and regulation of nifF from Rhodobacter capsulatus.
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Authors
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G.Gennaro,
P.Hübner,
U.Sandmeier,
A.F.Yakunin,
P.C.Hallenbeck.
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Ref.
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J Bacteriol, 1996,
178,
3949-3952.
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PubMed id
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Abstract
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The Rhodobacter capsulatus nifF gene and upstream sequence were cloned by using
a probe based on the N-terminal sequence of NifF. nifF was found to not be
contained in the previously described nif regions I, II, and III. Comparison of
the deduced amino acid sequence showed that it is highly similar to NifF from
Azotobacter vinelandii and NifF from Klebsiella pneumoniae. Analysis of
translational fusions demonstrated that the regulation of transcription was the
same as previously reported at the protein level. Insertional mutagen esis
showed that NifF contributes significantly to nitrogenase activity under normal
nitrogen-fixing conditions and that it is absolutely required for nitrogen
fixation under iron limitation.
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