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Simian immunodeficiency virus (SIV) proteinase has been crystallized from sodium
acetate buffer with sodium chloride as precipitant. The crystals are
orthorhombic and the space group is C222(1) with unit cell dimensions a = 32.18
A, b = 62.52 A, c = 95.76 A, alpha = beta = gamma = 90 degrees, indicating a
single monomer of 10 kDa in the asymmetric unit. The crystals grow to dimensions
of 0.2 mm x 0.2 mm x 0.07 mm within a week and are stable in the X-ray beam for
at least 50 hours. A different crystal lattice was observed for SIV proteinase
crystallized in the presence of pepstatin. The space group was P2(1)2(1)2(1)
with cell dimensions of a = 35.26 A, b = 58.59 A, c = 93.95 A, alpha = beta =
gamma = 90 degrees. Diffraction beyond 1.7 A was observed, indicating that a
high resolution structure analysis is feasible.
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