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Title
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Co-crystal structure of the HNF-3/fork head DNA-recognition motif resembles histone H5.
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Authors
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K.L.Clark,
E.D.Halay,
E.Lai,
S.K.Burley.
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Ref.
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Nature, 1993,
364,
412-420.
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PubMed id
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Abstract
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The three-dimensional structure of an HNF-3/fork head DNA-recognition motif
complexed with DNA has been determined by X-ray crystallography at 2.5 A
resolution. This alpha/beta protein binds B-DNA as a monomer, through
interactions with the DNA backbone and through both direct and water-mediated
major and minor groove base contacts, inducing a 13 degrees bend. The
transcription factor fold is very similar to the structure of histone H5. In its
amino-terminal half, three alpha-helices adopt a compact structure that presents
the third helix to the major groove. The remainder of the protein includes a
twisted, antiparallel beta-structure and random coil that interacts with the
minor groove.
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