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Title
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Preliminary crystallographic study of a complex between an Fab of a monoclonal feline peritonitis virus neutralizing antibody and its anti-idiotypic Fab.
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Authors
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N.Ban,
C.Escobar,
J.Day,
A.Greenwood,
A.McPherson.
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Ref.
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J Mol Biol, 1993,
234,
894-896.
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PubMed id
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Abstract
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A complex between an Fab fragment of an E2 specific feline infectious
peritonitis virus neutralizing antibody 730.1.4 and Fab fragment from
anti-idiotypic monoclonal antibody 409.5.3, was crystallized from ammonium
sulfate using vapor diffusion methods. The complex crystals diffract to about
2.9 A resolution and are of orthorhombic space group P2(1)22(1) with a = 75.2 A,
b = 80.6 A and c = 187.6 A. There are two Fab molecules, or one
idiotype-anti-idiotype complex, comprising the asymmetric unit. The long 187.6 A
c axis suggests that the long axis of the complex might lie along this
direction. Although small and radiation-sensitive, crystals are suitable for
three-dimensional structural analysis.
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