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Title
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Characterization of crystals of satellite panicum mosaic virus.
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Authors
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J.Day,
N.Ban,
S.Patel,
S.B.Larson,
A.McPherson.
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Ref.
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J Mol Biol, 1994,
238,
849-851.
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PubMed id
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Abstract
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Satellite panicum mosaic virus (SPMV) has been purified from pearl millet and
obtained in a variety of different crystal forms, at least two of which appear
suitable for high resolution X-ray diffraction analysis. The first is of cubic
space group P4(2)32 with a = b = c = 183.1 A and two virus particles in the unit
cell. The second is of a primitive orthorhombic space group with a = 166.1 A, b
= 266.7 A and c = 269.1 A, with four virus particles in the unit cell. While the
cubic crystal has as its asymmetric unit one twelfth of the icosahedron, or five
capsid protein subunits, the asymmetric unit of the orthorhombic crystals is an
entire particle. The cubic crystals diffract to at least 2.8 A resolution. We
have also succeeded in crystallizing, but not yet characterizing the master
virus, PMV.
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