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Title
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Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli.
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Authors
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A.A.Fedorov,
T.D.Pollard,
S.C.Almo.
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Ref.
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J Mol Biol, 1994,
241,
480-482.
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PubMed id
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Abstract
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Human platelet profilin was expressed in Escherichia coli using a T7 based
expression vector. The recombinant material is similar to authentic human
platelet profilin based on the measured Kd for rabbit skeletal muscle actin.
Crystals of the recombinant material were obtained from both PEG 8000 and
(NH4)2SO4. These crystals are isomorphous and belong to the monoclinic space
group C2, a = 75.0, b = 32.0, c = 62.5, beta = 123 degrees. These crystals
contain one molecule in the asymmetric unit and diffract to at least 2.0 A.
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