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Title
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Crystallization and preliminary X-ray diffraction studies of xylose isomerase from Thermoanaerobacterium thermosulfurigenes strain 4B.
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Authors
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L.F.Lloyd,
O.S.Gallay,
J.Akins,
J.G.Zeikus.
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Ref.
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J Mol Biol, 1994,
240,
504-506.
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PubMed id
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Abstract
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Xylose isomerase from the thermophile Thermoanaerobacterium thermosulfurigenes
strain 4B has been crystallized by vapour diffusion from Jeffamine ED 4000 as
precipitant. The crystal symmetry is P2(1)2(1)2(1), with unit cell dimensions a
= 85.6 A, b = 153.5 A and c = 158.5 A. The protein molecular mass and volume of
the unit cell is consistent with the presence of a tetramer of the enzyme in the
asymmetric unit. The crystals diffract X-rays from a synchrotron source to 1.7 A
resolution.
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