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Title
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X-ray structure analysis of a membrane protein complex. Electron density map at 3 A resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis.
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Authors
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J.Deisenhofer,
O.Epp,
K.Miki,
R.Huber,
H.Michel.
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Ref.
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J Mol Biol, 1984,
180,
385-398.
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PubMed id
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Abstract
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X-ray analysis of three-dimensional crystals of the photosynthetic reaction
center from the purple bacterium Rhodopseudomonas viridis led to an electron
density distribution at 3 A resolution calculated with phases from multiple
isomorphous replacement. The protein subunits of the complex were identified. An
atomic model of the prosthetic groups of the reaction center complex (4
bacteriochlorophyll b, 2 bacteriopheophytin b. 1 non-heme iron, 1 menaquinone, 4
heme groups) was built. The arrangement of the ring systems of the
bacteriochlorophyll b and bacteriopheophytin b molecules shows a local 2-fold
rotation symmetry; two bacteriochlorophyll b form a closely associated,
non-covalently linked dimer ("special pair"). A different local 2-fold
symmetry axis is observed for the heme groups of the cytochrome part.
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