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Title
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Crystallographic studies of the chicken gizzard G-actin X DNase I complex at 5A resolution.
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Authors
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N.Sakabe,
K.Sakabe,
K.Sasaki,
H.Kondo,
T.Ema,
N.Kamiya,
M.Matsushima.
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Ref.
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J Biochem (tokyo), 1983,
93,
299-302.
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PubMed id
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Abstract
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The structure of the chicken gizzard G-actin X DNase I complex has been
determined at 5 A resolution by an X-ray diffraction method. Protein phases were
computed by the multiple isomorphous replacement method using four heavy atom
derivatives. The mean figure of merit was 0.65. Dimensions of the three
molecular species, the complex, G-actin and DNase I, were determined based on
the "cypress wood" models derived from the electron density map. The natures of
the heavy atom binding sites are discussed in relation to the distinction
between the two component molecules. The pattern of successive contacts between
actin molecules observed in the present crystal seems unrelated to that found in
F-actin.
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