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Title
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Crystallization and preliminary crystallographic data of chicken gizzard G-actin . DNase I complex and Physarum G-actin . DNase I complex.
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Authors
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H.Sugino,
N.Sakabe,
K.Sakabe,
S.Hatano,
F.Oosawa,
T.Mikawa,
S.Ebashi.
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Ref.
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J Biochem (tokyo), 1979,
86,
257-260.
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PubMed id
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Abstract
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Smooth muscle G-actin from chicken gizzard and Physarum plasmodium G-actin both
interact with DNase I and form 1 : 1 complexes. These complexes were
crystallized by using polyethylene glycol 6000 as a precipitant. Both crystals
belong to the same orthorhombic space group P2(1)2(1)2(1). The cell dimensions
of chicken gizzard G-actin.DNase I complex are a=42.00 +/- 0.07 A, b=225.3 +/-
0.4 A, and c=77.4 +/- 0.1 A, while those of Physarum G-actin.DNase I complex are
a=42 A, b=221 A, and c=77 A.
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