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Title
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The catalytic mechanism of Escherichia coli aspartate carbamoyltransferase: a molecular modelling study.
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Authors
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J.E.Gouaux,
K.L.Krause,
W.N.Lipscomb.
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Ref.
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Biochem Biophys Res Commun, 1987,
142,
893-897.
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PubMed id
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Abstract
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Based on molecular modelling study, we propose that the reaction between
L-aspartate an carbamoylphosphate, catalyzed by E. coli aspartate
carbamoyltransferase, may proceed via a tetrahedral intermediate and that the
breakdown of the intermediate is facilitated by an intramolecular proton
transfer between the amino group of L-aspartate and a terminal phosphate oxygen
of carbamoylphosphate.
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