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Title
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Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10.
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Authors
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H.J.Rozeboom,
J.Kingma,
D.B.Janssen,
B.W.Dijkstra.
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Ref.
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J Mol Biol, 1988,
200,
611-612.
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PubMed id
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Abstract
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Haloalkane dehalogenases are enzymes that release chloride or bromide from
n-halogenated alkanes. X-ray quality crystals of haloalkane dehalogenase from
the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 have
been grown at room temperature from 64% saturated ammonium sulfate solutions (pH
6.2 to 6.4). The crystals diffract in the X-ray beam to at least 2.4 A
resolution (1 A = 0.1 nm). Their space group is P2(1)2(1)2, with cell dimensions
a = 94.1 A, b = 72.8 A, c = 41.4 A and alpha = beta = gamma = 90 degrees. There
is one monomer (molecular weight 36,000) per asymmetric unit.
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