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Title
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Analysis of an anti-progesterone antibody: variable crystal morphology of the Fab' and steroid-Fab' complexes.
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Authors
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E.A.Stura,
J.H.Arevalo,
A.Feinstein,
R.B.Heap,
M.J.Taussig,
I.A.Wilson.
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Ref.
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Immunology, 1987,
62,
511-521.
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PubMed id
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Abstract
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Anti-progesterone monoclonal antibodies are being used for structural studies of
antibody-antigen interaction, for their ability to block pregnancy shortly after
fertilization, and for hormone immunoassay. A mouse anti-progesterone monoclonal
Fab' fragment has been crystallized in its native form and co-crystallized with
seven different, but structurally related, steroids. The crystals show
interesting preferences in their crystal morphology, depending on the bound
steroid ligand. The X-ray crystallographic analysis of this Fab', complexed with
a series of related steroid ligands, should reveal details of the chemistry of
antibody-antigen union and provide insights into how steroids interact with
proteins.
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