 |
|
Title
|
|
Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa.
|
|
|
Authors
|
|
D.H.Ohlendorf,
P.C.Weber,
J.D.Lipscomb.
|
|
|
Ref.
|
|
J Mol Biol, 1987,
195,
225-227.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
A 2.5 A resolution data set has been collected for crystals of protocatechuate
3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the
rotation function shows that the alpha 2 beta 2 tetramers associate to form a
particle with cubic 23 (T) point group symmetry. Prior to this analysis it was
believed that eight tetramers associated to form the holoenzyme. The symmetry of
the crystalline holoenzyme also addresses questions concerning its iron content
and substrate stoichiometry.
|
|
|
|