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Title
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Relative effectiveness of various ions on the solubility and crystal growth of lysozyme.
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Authors
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M.M.Ries-Kautt,
A.F.Ducruix.
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Ref.
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J Biol Chem, 1989,
264,
745-748.
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PubMed id
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Abstract
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Crystallization conditions for hen egg white lysozyme in the presence of various
ions were determined at pH 4.5 and 18 degrees C. The corresponding solubility
curves show that the main effects are due to anions in the following order: SCN-
greater than NO3- greater than Cl- greater than citrate2- greater than CH3COO-
approximately H2PO4- greater than SO4(2-). This is in the reverse order of the
lyotropic series of Hofmeister. As a consequence, SCN- precipitates and
crystallizes lysozyme at low concentration, whereas sulfate is ineffective even
at high concentrations. Crystals obtained with each salt were characterized by
x-ray diffraction. Lysozyme thiocyanate and nitrate crystals belong to the
monoclinic system, whereas all the others have a tetragonal lattice.
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