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Title
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Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation.
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Authors
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M.L.Cleary,
S.D.Smith,
J.Sklar.
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Ref.
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Cell, 1986,
47,
19-28.
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PubMed id
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Abstract
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cDNAs for the bcl-2 mRNA were cloned from a human common acute lymphoblastic
leukemia cell line. Nucleotide sequence analyses showed that the 6 kb bcl-2 mRNA
potentially encodes a 26 kd protein that is homologous to a predicted
Epstein-Barr virus protein. Most t(14;18) translocation breakpoints cluster
within a narrow region of a 5.4 kb exon that contains a long 3'-untranslated
region of the bcl-2 mRNA. As a result of t(14;18) translocation, hybrid
bcl-2/immunoglobulin heavy chain transcripts are produced that consist of the 5'
half of the bcl-2 mRNA fused to a "decapitated" immunoglobulin heavy
chain mRNA. Nucleotide sequence analyses confirmed that the hybrid transcripts
continue to encode a normal bcl-2 protein. Our results suggest that t(14;18)
translocations alter expression of the bcl-2 gene both by transcriptional
activation and by abnormal posttranscriptional regulation of bcl-2 mRNA.
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