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Title
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Preliminary crystallographic analysis of a plant pathogenic factor: pectate lyase.
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Authors
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C.Y.Kim,
V.Mosser,
N.Keen,
F.Jurnak.
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Ref.
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J Mol Biol, 1989,
208,
365-367.
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PubMed id
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Abstract
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Pectate lyase is a saccharide-binding enzyme that lyitically depolymerizes
polypectate in higher plant cell walls, thus causing soft-rot diseases in food
crops. A pectate lyase from Erwinia chrysanthemi, EC16 (PLe), crystallizes in
the orthorhombic space group P2(1)2(1)2(1) with unit cell dimension of a = 39.0
A, b = 91.0 A and c = 103.4 A. The asymmetric unit consists of one molecule with
a molecular mass of 38,118 daltons and the X-ray diffraction extends to a
resolution of 1.8 A. The crystals reproducibly grow to large dimensions and are
suitable for a high-resolution X-ray diffraction analysis.
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