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Title
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Crystallization and preliminary X-ray studies of glutathione synthetase from Escherichia coli B.
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Authors
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H.Kato,
H.Yamaguchi,
Y.Hata,
T.Nishioka,
Y.Katsube,
J.Oda.
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Ref.
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J Mol Biol, 1989,
209,
503-504.
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PubMed id
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Abstract
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The glutathione synthetase from Escherichia coli B has been crystallized from
27% saturated ammonium sulfate solution (pH 5.5). The crystals are hexagonal,
space group P6(2)22 or P6(4)22. The cell dimensions are a = b = 88.0 A, c =
164.2 A, and gamma = 120 degrees. The enzyme is a tetramer (Mr = 143,000) with
222 symmetry, and the asymmetric unit contains one subunit molecule (Mr =
35,600). The crystals diffract to at least 2.5 A resolution.
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