 |
|
Title
|
|
Amino acid sequence of the bacterioferritin (cytochrome b1) of Escherichia coli-K12.
|
|
|
Authors
|
|
S.C.Andrews,
J.M.Smith,
J.R.Guest,
P.M.Harrison.
|
|
|
Ref.
|
|
Biochem Biophys Res Commun, 1989,
158,
489-496.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The complete amino acid sequence of bacterioferritin (cytochrome b1) from
Escherichia coli-K12 has been derived from the nucleotide sequence of the cloned
gene. It comprises 158 amino acid residues giving an Mr of 18,495. The identity
of the gene product was confirmed by an 87 residue N-terminal sequence obtained
from the purified protein, but it differs significantly from much of the
previously published partial amino acid sequence (1). Secondary structure
prediction indicates a high alpha-helical content consistent with a
4-helix-bundle conformation. The fully assembled bacterioferritin molecule
comprising 24 identical subunits and 12 haem moieties is a tetracosamer with an
Mr of approximately 452,000.
|
|
|
|