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Title
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Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src.
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Authors
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S.K.Anderson,
C.P.Gibbs,
A.Tanaka,
H.J.Kung,
D.J.Fujita.
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Ref.
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Mol Cell Biol, 1985,
5,
1122-1129.
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PubMed id
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Abstract
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The nucleotide sequence of the 3' two-thirds of a highly conserved, molecularly
cloned human cellular src gene (c-src) has been determined. This region of the
c-src gene encodes the tyrosine kinase domain of the cellular src protein
(pp60c-src) and corresponds to exons 6 through 12 of the chicken c-src gene, as
well as nucleotides 545 to 1542 of the Rous sarcoma virus src gene (v-src). The
human c-src sequence is very strongly conserved with respect to both the chicken
c-src and the Rous sarcoma virus v-src genes, with nearly 90% nucleotide
homology observed in this region. Amino acid sequence conservation in this
region is even greater; 98% of the amino acids are conserved between human and
chicken c-src. Furthermore, the exon sizes and the locations of the exon-intron
boundaries are identical in the human and chicken c-src genes. However,
sequences within the introns have not been conserved, and the introns within the
human c-src gene are significantly larger than the corresponding introns within
the chicken c-src gene. The strong amino acid conservation between the
carboxy-terminal two-thirds of pp60c-src of species as divergent as humans and
chickens suggests that this portion of the pp60c-src protein specifies one or
more functional domains that are of great importance to some aspect of normal
cellular growth or differentiation.
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