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Title
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Spectroscopic investigations of Panulirus interruptus hemocyanin in the crystalline state.
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Authors
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A.Volbeda,
M.C.Feiters,
M.G.Vincent,
E.Bouwman,
B.Dobson,
K.H.Kalk,
J.Reedijk,
W.G.Hol.
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Ref.
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Eur J Biochem, 1989,
181,
669-673.
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PubMed id
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Abstract
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Single-crystal ultraviolet spectroscopy, X-ray absorption spectroscopy and EPR
measurements have been used to examine the oxidation and oxygenation state of
the dinuclear copper site of several types of hemocyanin crystals. The crystals
contain Panulirus interruptus hemocyanin which forms hexameric molecules with a
molecular mass of approximately 470 kDa. Three types of crystals have been
investigated. Type-I monoclinic crystals, which have been used for the X-ray
structure determination, contain virtually only deoxyhemocyanin. Type-II
monoclinic crystals, which are less well ordered than the type-I crystals,
contain a mixture of deoxy, oxy and met forms. Older crystals contain relatively
more methemocyanin. A third, hexagonal, crystal form is also partially
oxygenated, and, like the type-II monoclinic form, subject to gradual conversion
to methemocyanin.
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