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Title
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cDNA cloning, primary structure and gene expression for H-protein, a component of the glycine-cleavage system (glycine decarboxylase) of pea (Pisum sativum) leaf mitochondria.
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Authors
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D.Macherel,
M.Lebrun,
J.Gagnon,
M.Neuburger,
R.Douce.
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Ref.
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Biochem J, 1990,
268,
783-789.
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PubMed id
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Abstract
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We have isolated and characterized cDNA clones encoding the H-protein of the
glycine-cleavage system of pea (Pisum sativum) leaf mitochondria. The deduced
primary structure revealed that the 131-amino-acid polypeptide is
cytoplasmically synthesized with a 34-amino-acid mitochondrial targeting
peptide. The lipoate-binding site was assigned to be lysine-63, as deduced from
a sequence comparison with several lipoate-bearing proteins. The expression of
the gene encoding H-protein was shown to occur specifically in the leaf tissue,
with light exerting an additional effect by increasing the mRNA levels
severalfold. Two polyadenylation sites were found in the mRNA, and a single-copy
gene encoding the H-protein was detected in pea genome.
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