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Title
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Engineering of microheterogeneity-resistant p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
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Authors
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K.Eschrich,
W.J.van Berkel,
A.H.Westphal,
A.de Kok,
A.Mattevi,
G.Obmolova,
K.H.Kalk,
W.G.Hol.
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Ref.
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FEBS Lett, 1990,
277,
197-199.
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PubMed id
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Abstract
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By site-directed mutagenesis, Cys-116 was converted to Ser-116 in
p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens. In
contrast to wild-type enzyme, the C116S mutant is no longer susceptible to
oxidation by hydrogen peroxide and shows no reactivity towards
5,5'-dithiobis(2-nitrobenzoate). Crystals of the C116S mutant are isomorphous
with the crystal form of wild-type enzyme. A difference electron density
confirms the mutation made.
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