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Title
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Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
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Authors
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F.Lévêque,
P.Plateau,
P.Dessen,
S.Blanquet.
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Ref.
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Nucleic Acids Res, 1990,
18,
305-312.
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PubMed id
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Abstract
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In Escherichia coli, two distinct lysyl-tRNA synthetase species are encoded by
two genes: the constitutive lysS gene and the thermoinducible lysU gene. These
two genes have been isolated and sequenced. Their nucleotide and deduced amino
acid sequences show 79% and 88% identity, respectively. Codon usage analysis
indicates the lysS product being more efficiently translated than the lysU one.
In addition, the lysS sequence exactly coincides with the sequence of herC, a
gene which is part of the prfB-herC operon. In contrast to the recent proposal
of Gampel and Tzagoloff (1989, Proc. Natl. Acad. Sci. USA 86, 6023-6027), the
lysU sequence is distinct from the open reading frame located adjacent to frdA,
although large homologies are shared by these two genes.
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