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Title
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Crystallization and preliminary crystallographic analysis of the Clostridium perfringens enterotoxin.
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Authors
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D.C.Briggs,
J.G.Smedley,
B.A.McClane,
A.K.Basak.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2010,
66,
794-797.
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PubMed id
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Abstract
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Clostridium perfringens is a Gram-positive anaerobic species of bacterium that
is notable for its ability to produce a plethora of toxins, including
membrane-active toxins (alpha-toxins), pore-forming toxins (-toxins) and binary
toxins (iota-toxins). Here, the crystallization of the full-length wild-type C.
perfringens enterotoxin is reported, which is the causative agent of the second
most prevalent food-borne illness in the United States and has been implicated
in many other gastrointestinal pathologies. Several crystal forms were obtained.
However, only two of these optimized crystal forms (I and II) were useable for
X-ray diffraction data collection. The form I crystals diffracted to d(min) =
2.7 A and belonged to space group C2, while the form II crystals diffracted to
d(min) = 4 A and belonged to space group P2(1)3.
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