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Title
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Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7.
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Authors
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T.Kumarevel,
T.Tanaka,
M.Nishio,
S.C.Gopinath,
K.Takio,
A.Shinkai,
P.K.Kumar,
S.Yokoyama.
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Ref.
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J Struct Biol, 2008,
161,
9.
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PubMed id
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Abstract
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The emergence of bacterial resistance to multiple drugs poses a serious and
growing health concern. Understanding and deciphering the mechanisms of these
multiple drug resistance regulatory proteins through structural or biochemical
means is an important endeavor. Here, we present the crystal structure of ST1710
from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and
2.0A, respectively. The overall structure of the ST1710 dimer shares the
topology of the MarR family of proteins, with each subunit containing a winged
helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by
biochemical methods. Our molecular modeling analysis suggested that Asp88 and
Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.
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