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Title
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The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control.
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Authors
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A.N.Myasnikov,
K.V.Sasnauskas,
A.A.Janulaitis,
M.N.Smirnov.
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Ref.
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Gene, 1991,
109,
143-147.
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PubMed id
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Abstract
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The ADE1 gene of the yeast Saccharomyces cerevisiae has been cloned by
complementation of the ade1 mutation. The nucleotide sequence has been
determined for the 918-bp coding region, 240-bp 5'-noncoding region and 292-bp
3'-noncoding region. The sequenced region includes a single large open reading
frame coding for a protein of 306 amino acid (aa) residues. The promoter of the
ADE1 gene contains a copy of the 5'-TGACTC hexanucleotide, a feature
characteristic of promoters under general aa control. Subsequent search of other
published purine biosynthesis gene sequences revealed that all of them also
contain general aa control signals in their promoter regions. An expression
plasmid containing the ADE1 coding region under control of the PHO5 promoter
produced N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase
in yeast cells at a level of 40% of total cellular protein. One-step
purification resulted in an almost homogeneous preparation of SAICAR synthetase.
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