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Title
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1H, 13C and 15N backbone assignments of cyclophilin when bound to cyclosporin A (CsA) and preliminary structural characterization of the CsA binding site.
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Authors
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P.Neri,
R.Meadows,
G.Gemmecker,
E.Olejniczak,
D.Nettesheim,
T.Logan,
R.Simmer,
R.Helfrich,
T.Holzman,
J.Severin.
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Ref.
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FEBS Lett, 1991,
294,
81-88.
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PubMed id
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Abstract
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The backbone 1H, 13C and 15N chemical shifts of cyclophilin (CyP) when bound to
cyclosporin A (CsA) have been assigned from heteronuclear two- and
three-dimensional NMR experiments involving selectively 15N- and uniformly 15N-
and 15N,13C-labeled cyclophilin. From an analysis of the 1H and 15N chemical
shifts of CyP that change upon binding to CsA and from CyP/CsA NOEs, we have
determined the regions of cyclophilin involved in binding to CsA.
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