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Mitogillin is a ribonuclease secreted by the fungus Aspergillus restrictus. The
substrate for mitogillin is a short, universally conserved, sequence in
ribosomal RNA. Cleavage of this sequence inactivates protein synthesis.
Mitogillin was crystallized by a two-chamber vapor/liquid diffusion method using
ethanol as the precipitant. This method has wider potential in the use of
volatile organic solvents as precipitants. Crystals of mitogillin diffract
X-rays to lattice d-spacings of at least 1.6 A, and belong to the monoclinic
space group P2(1), with a = 50.4 A, b = 82.4 A, c = 38.2 A and beta = 99.8
degrees.
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