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Title
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Crystallization and preliminary X-ray analysis of Atg3.
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Authors
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Y.Yamada,
N.N.Suzuki,
Y.Fujioka,
Y.Ichimura,
Y.Ohsumi,
F.Inagaki.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2006,
62,
1016-1017.
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PubMed id
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Abstract
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Atg3 is an E2-like enzyme that catalyzes the conjugation reaction between Atg8
and phosphatidylethanolamine (PE). The Atg8-PE conjugate is essential for
autophagy, the bulk degradation process of cytoplasmic components by the
vacuolar/lysosomal system. Crystals of Saccharomyces cerevisiae Atg3 have been
obtained by the sitting-drop vapour-diffusion method using ammonium sulfate and
lithium sulfate as precipitants. A native data set was collected from a single
crystal to 2.5 A resolution. The crystals belong to space group P4(1) or P4(3),
with unit-cell parameters a = 59.33, c = 115.22 A, and are expected to contain
one protein molecule per asymmetric unit.
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