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Title
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Characterization of crystals of an Fab fragment of a murine monoclonal antibody.
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Authors
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N.Ban,
C.Escobar,
J.Day,
A.Greenwood,
S.Larson,
A.McPherson.
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Ref.
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J Mol Biol, 1991,
222,
445-446.
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PubMed id
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Abstract
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The Fab fragment of an antibody, made against an E2-specific feline infectious
peritonitis virus neutralizing antibody, has been crystallized in a form
suitable for X-ray diffraction analysis from PEG 4000 using vapor diffusion
methods. The Fab fragment crystals diffract to about 2.9 A resolution and are of
triclinic space group P1. Unit cell dimensions, by which the reciprocal lattice
can be indexed, are a = 57.16 A, b = 70.85 A, c = 75.81 A, alpha = 85.11
degrees, beta = 121.28 degrees and gamma = 116.33 degrees. There are two Fab
fragments comprising the asymmetric unit of the crystals. The presence of a
pseudo-mirror plane in the diffraction pattern suggests the presence of at least
an approximate dyad axis relating the two Fab fragments within the asymmetric
unit.
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