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Title
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Crystallization and preliminary X-ray analysis of reducing-end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125.
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Authors
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Y.Honda,
S.Fushinobu,
M.Hidaka,
T.Wakagi,
H.Shoun,
M.Kitaoka.
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Ref.
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Acta Crystallograph Sect F Struct Biol Cryst Commun, 2005,
61,
291-292.
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PubMed id
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Abstract
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The reducing-end xylose-releasing exo-oligoxylanase (Rex) from Bacillus
halodurans C-125, a novel family GH8 glycoside hydrolase, was crystallized by
the hanging-drop vapour-diffusion method using 13.6 mg ml(-1) purified Rex,
5.6%(v/v) polyethylene glycol 4000, 70 mM sodium acetate pH 4.6 and 30%(v/v)
glycerol. Suitable crystals grew after incubation for 5 d at 293 K. The crystals
belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.69, b =
86.02, c = 87.92 A. X-ray diffraction data were collected at a resolution of
1.35 A.
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