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Title
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Crystallization and preliminary X-ray analysis of pig kidney DOPA decarboxylase.
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Authors
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V.N.Malashkevich,
P.Filipponi,
U.Sauder,
P.Dominici,
J.N.Jansonius,
C.Borri Voltattorni.
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Ref.
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J Mol Biol, 1992,
224,
1167-1170.
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PubMed id
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Abstract
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DOPA decarboxylase from pig kidney, an alpha 2 dimeric enzyme of Mr = 107,000,
has been crystallized by the vapour diffusion method with ammonium sulphate as
precipitant. The crystals belong to the space group P6(2) (or its enantiomer
P6(4)) and have unit cell dimensions of a = b = 155.9 A, c = 87.7 A, alpha =
beta = 90 degrees, gamma = 120 degrees. They diffract to 2.6 A resolution. There
is one dimeric molecule per asymmetric unit. Rotation function studies have
revealed the orientation of the non-crystallographic 2-fold axis of the dimer in
the asymmetric unit.
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