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X-ray quality crystals of a soluble murine class I H-2Kb molecule complexed with
three different peptide antigens were grown in several forms by streak seeding
and macroseeding methods. Co-crystals with VSV-8 (RGYVYGQL), OVA-8 (SIINFEKL)
and SEV-9 (FAPGNYPAL) peptides were grown either from NaH2PO4/HPO4 or from
polyethylene glycol 4000 within the pH range 5.0 to 7.5, with the use of
4-methyl-2-pentane diol (MPD) as an additive. The VSV-8 crystals grew in space
groups P1, with cell dimensions a = 63.1 A, b = 69.1 A, c = 72.0 A, alpha = 89.9
degrees, beta = 77.1 degrees, gamma = 123.3 degrees and P2(1)2(1)2, with a =
138.1 A, b = 88.6 A, c = 45.7 A, and diffract to 2.9 and 2.3 A, respectively.
Crystals of the SEV-9 complex grew from similar crystallization conditions to
those of the orthorhombic VSV-8 complex with similar cell parameters and
diffract to at least 2.5 A resolution. Crystals of the OVA-8 complex were
obtained from either phosphate (space group C2, a = 118.7 A, b = 61.6 A, c =
85.3 A, beta = 108.4 degrees) or polyethylene glycol (space group P1, a = 64.5
A, b = 71.0 A, c = 66.3 A, alpha = 89.7 degrees, beta = 95.7 degrees, gamma =
123.3 degrees) and diffract to 3 A resolution. The crystallization procedures
used here significantly increased the rate and production of X-ray quality
crystals.
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