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Title
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Crystallization of the oligopeptide-binding protein AppA from Bacillus subtilis.
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Authors
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L.Wright,
E.Blagova,
V.M.Levdikov,
J.A.Brannigan,
R.J.Pattenden,
J.Chambers,
A.J.Wilkinson.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2004,
60,
175-177.
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PubMed id
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Abstract
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AppA is the membrane-anchored extracellular receptor component of an ABC
transporter responsible for the uptake of oligopeptides into Bacillus subtilis.
AppA has been overexpressed as a cleavable maltose-binding protein fusion in
Escherichia coli. Following removal of the fusion portion, AppA has been
crystallized from morpholinoethanesulfonic acid-buffered solutions at pH 6.5
containing polyethylene glycol and zinc acetate. A complete X-ray diffraction
data set extending to 2.3 A spacing has been collected.
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