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Title
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Crystallization and preliminary X-ray investigation of phosphoribosylaminoimidazolesuccinocarboxamide synthase from the yeast Saccharomyces cerevisiae.
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Authors
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A.I.Grebenko,
V.M.Levdikov,
V.V.Barynin,
W.R.Melik-Adamyan,
A.N.Myasnikov.
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Ref.
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J Mol Biol, 1992,
228,
298-299.
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PubMed id
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Abstract
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Crystals of phosphoribosylaminoimidazolesuccinocarboxamide synthase (EC 6.3.2.6)
from the yeast Saccharomyces cerevisiae were grown by the vapor diffusion
hanging-drop technique, using ammonium sulfate as the precipitant. The crystals
had dimensions up to 1.2 mm. X-ray diffraction experiments indicated a space
group of P2(1)2(1)2(1) and unit cell parameters of a = 62.3 A, b = 63.5 A and c
= 80.9 A, with one molecule in the asymmetric unit. Native data have been
collected to 2.5 A resolution.
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