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Title
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Structure of a C-type mannose-binding protein complexed with an oligosaccharide.
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Authors
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W.I.Weis,
K.Drickamer,
W.A.Hendrickson.
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Ref.
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Nature, 1992,
360,
127-134.
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PubMed id
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Abstract
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C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins
mediate many cell-surface carbohydrate-recognition events. The crystal structure
at 1.7 A resolution of the carbohydrate-recognition domain of rat
mannose-binding protein complexed with an oligomannose
asparaginyl-oligosaccharide reveals that Ca2+ forms coordination bonds with the
carbohydrate ligand. Carbohydrate specificity is determined by a network of
coordination and hydrogen bonds that stabilizes the ternary complex of protein,
Ca2+ and sugar. Two branches of the oligosaccharide crosslink neighbouring
carbohydrate-recognition domains in the crystal, enabling multivalent binding to
a single oligosaccharide chain to be visualized directly.
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