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Title
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Overproduction, crystallization, and preliminary X-ray diffraction studies of the major cold shock protein from Bacillus subtilis, CspB.
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Authors
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H.Schindelin,
M.Herrler,
G.Willimsky,
M.A.Marahiel,
U.Heinemann.
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Ref.
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Proteins, 1992,
14,
120-124.
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PubMed id
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Abstract
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The major cold shock protein from Bacillus subtilis (CspB) was overexpressed
using the bacteriophage T7 RNA polymerase/promoter system and purified to
apparent homogeneity from recombinant Escherichia coli cells. CspB was
crystallized in two different forms using vapor diffusion methods. The first
crystal form obtained with ammonium sulfate as precipitant belongs to the
trigonal crystal system, space group P3(1)21 (P3(2)21) with unit cell dimensions
a = b = 59.1 A and c = 46.4 A. The second crystal form is tetragonal, space
group P4(1)2(1)2 (P4(3)2(1)2) with unit cell dimensions a = b = 56.9 A and c =
53.0 A. These crystals grow with polyethylene glycol 4000 as precipitant.
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