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Title
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cDNA cloning of human and rat brain myo-inositol monophosphatase. Expression and characterization of the human recombinant enzyme.
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Authors
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G.McAllister,
P.Whiting,
E.A.Hammond,
M.R.Knowles,
J.R.Atack,
F.J.Bailey,
R.Maigetter,
C.I.Ragan.
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Ref.
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Biochem J, 1992,
284,
749-754.
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PubMed id
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Abstract
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Inositol monophosphatase (EC 3.1.3.25) is a key enzyme in the phosphoinositide
cell-signalling system. Its role is to provide inositol required for the
resynthesis of phosphatidylinositol and polyphosphoinositides. It is the
probable pharmacological target for lithium action in brain. Using probes
derived from the bovine inositol monophosphatase cDNA we have isolated cDNA
clones encoding the human and rat brain enzymes. The enzyme is highly conserved
in all three species (79% identical). The coding region of the human cDNA was
inserted into a bacterial expression vector. The expressed recombinant enzyme
was purified and its biochemical properties examined. The human enzyme is very
similar to the bovine enzyme.
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