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Title
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Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.
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Authors
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T.Zink,
A.Ross,
D.Ambrosius,
R.Rudolph,
T.A.Holak.
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Ref.
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FEBS Lett, 1992,
314,
435-439.
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PubMed id
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Abstract
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Recombinant 15N-, 13C-labeled human granulocyte colony-stimulating factor
(rh-metG-CSF) has been studied by 2D and 3D NMR using uniformly labeled protein
as well as residue-specific 15N-labeled samples. Assignment of the 1H, 15N
backbone, and 60% 1H sidechain resonances has enabled the determination of the
secondary structure of the protein. The secondary structure is dominated by
alpha-helical regions with four stretches of helices between residues 11-41,
71-95, 102-124 and 144-170.
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