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Title
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Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings.
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Authors
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A.Okamoto-Nakazato,
K.Takahashi,
R.Katoh-Semba,
K.Katou.
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Ref.
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Plant Cell Physiol, 2001,
42,
952-958.
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PubMed id
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Abstract
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We examined the distribution and the immunohistochemical localization of yieldin
in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting
analysis revealed that the yieldin was located in the aerial organs (plumule,
epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin
signal in the hypocotyls was highest in the apical pre-elongation region (the
hook region) and decreased toward the elongated mature base indicating that the
yieldin disappeared with the ceasing of cell elongation. Tissue-print
immunoblotting analysis using hypocotyls in different germination stages
supports this view because the apical yieldin-rich regions, just beneath the
cotyledonary node (the hook and rapidly elongating regions), acropetally
migrated together with hypocotyl elongation. Immunohistochemical microscopy
demonstrated that yieldin was localized in the cell walls of the cortex and
epidermis of the germ axes. The present results are consistent with the view
that yieldin participates in the regulation of cell wall yielding during
elongation growth.
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