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Title
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Crystallization and preliminary X-ray analysis of a chitinase from the fungal pathogen Coccidioides immitis.
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Authors
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T.Hollis,
A.F.Monzingo,
K.Bortone,
E.Schelp,
R.Cox,
J.D.Robertus.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1998,
54,
1412-1413.
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PubMed id
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Abstract
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Chitinase is necessary for fungal growth and cell division and, therefore, is an
ideal target for the design of inhibitors which may act as antifungal agents. A
chitinase from the fungal pathogen Coccidioides immitis has been expressed as a
fusion protein with gluathione-S-transferase (GST), which aids in purification.
After cleavage from GST, chitinase was crystallized from 30% PEG 4000 in 0. 1 M
sodium acetate pH 4.6. The crystals have a tetragonal crystal lattice and belong
to space group P41212 or P43212 and diffract to 2. 2 A resolution. The unit-cell
parameters are a = b = 91.2, c = 95.4 A; there is only one chitinase molecule in
the asymmetric unit.
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