PDBsum entry 9k0k

Membrane protein

PDB id: 9k0k

Contents

Protein chains

211 a.a.

327 a.a.

36 a.a.

126 a.a.

296 a.a.

Ligands

UTP

PDB id:

9k0k

Name:

Membrane protein

Title:

Cryo-em structure of utp-bound p2y purinoceptor 4-minigq-nb35 complex

Structure:

Guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: a. Synonym: adenylate cyclase-stimulating g alpha protein. Engineered: yes. Other_details: the n-terminal sequence of gi1 is incorporated into the mini-g protein.Certain residues mutated to match guanine nucleotide-binding protein g(q) subunit. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnas, gnas1, gsp. Expressed in: homo sapiens. Expression_system_taxid: 9606. Gene: gnb1. Expressed in: trichoplusia ni. Expression_system_taxid: 7111.

Authors:

B.Lan,S.Zhang,X.Liu,B.Lin

Key ref:

B.Lan et al. (2025). Structural insight into the self-Activation and g-Pro coupling of p2y2 receptor.. Cell discov, 11, 47. PubMed id: 40360475

Date:

15-Oct-24 Release date: 11-Jun-25

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 211 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 327 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 36 a.a.

Protein chain

No UniProt id for this chain

Struc: 126 a.a.

Protein chain

P51582  (P2RY4_HUMAN) -  P2Y purinoceptor 4 from Homo sapiens

Seq: 365 a.a.

Struc: 296 a.a.

* PDB and UniProt seqs differ at 61 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????