PDBsum entry 9izh

Membrane protein

PDB id: 9izh

Contents

Protein chains

299 a.a.

212 a.a.

339 a.a.

59 a.a.

232 a.a.

Ligands

NKP

PDB id:

9izh

Name:

Membrane protein

Title:

Cryo-em structure of lpa1-g13 complex with lpa

Structure:

Soluble cytochrome b562,lysophosphatidic acid receptor 1, lgbit tag. Chain: r. Synonym: cytochrome b-562,lpa receptor 1,lpa-1,lysophosphatidic acid receptor edg-2. Engineered: yes. Other_details: n-terminal ha signal sequence, flag tag c-terminal lgbit. G protein subunit 13 (gi2-mini-g13 chimera).

Source:

Escherichia coli, homo sapiens, synthetic construct. Human. Organism_taxid: 562, 9606, 32630. Gene: cybc, lpar1, edg2, lpa1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Homo sapiens. Organism_taxid: 9606.

Authors:

S.Suzuki,K.Nishikawa,A.Kamegawa,Y.Hiroaki,H.Suzuki,Y.Fujiyoshi

Key ref:

S.Suzuki et al. Structural insights into the engagement of lysophosph acid receptor 1 with different g proteins. To be published, . PubMed id: 39725093

Date:

01-Aug-24 Release date: 01-Jan-25

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 299 a.a.*

Protein chain

Q92633  (LPAR1_HUMAN) -  Lysophosphatidic acid receptor 1 from Homo sapiens

Seq: 364 a.a.

Struc: 299 a.a.

Protein chain

Q14344  (GNA13_HUMAN) -  Guanine nucleotide-binding protein subunit alpha-13 from Homo sapiens

Seq: 377 a.a.

Struc: 212 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P63213  (GBG2_MOUSE) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Mus musculus

Seq: 71 a.a.

Struc: 59 a.a.

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

* PDB and UniProt seqs differ at 152 residue positions (black crosses)