PDBsum entry 9hc0

Signaling protein

PDB id: 9hc0

Contents

Protein chain

414 a.a.

Ligands

OLA ×5

4YI

Waters ×22

PDB id:

9hc0

Name:

Signaling protein

Title:

Dark structure of the human metabotropic glutamate receptor 5 transmembrane domain bound to photoswitchable ligand alloswitch-1

Structure:

Metabotropic glutamate receptor 5,endolysin. Chain: a. Synonym: mglur5,lysis protein,lysozyme,muramidase. Engineered: yes. Mutation: yes. Other_details: metabotropic glutamate receptor 5 with t4 lysozyme insertion and thermostabilising mutations e579a, n667y, i669a, g675m, t742a, s753a

Source:

Homo sapiens, enterobacteria phage t4. Human, bacteriophage t4. Organism_taxid: 9606, 10665. Gene: grm5, gprc1e, mglur5, e. Expressed in: trichoplusia ni. Expression_system_taxid: 7111

Resolution:

2.33Å R-factor: 0.233 R-free: 0.271

Authors:

Y.Kondo,C.Hatton,R.Cheng,M.Trabuco,H.Glover,Q.Bertrand,F.Stierli, H.P.Seidel,T.Mason,S.Sarma,F.Tellkamp,M.Kepa,F.Dworkowski,P.Mehrabi, M.Hennig,J.Standfuss

Date:

08-Nov-24 Release date: 25-Jun-25

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 414 a.a.*

Protein chain

P41594  (GRM5_HUMAN) -  Metabotropic glutamate receptor 5 from Homo sapiens

Seq: 1212 a.a.

Struc: 414 a.a.*

* PDB and UniProt seqs differ at 174 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.